Testicular Injection of Autologous Platelet-Rich Plasma (PRP) to Enhance the Sperm Parameters in Rabbit

Alaa Kamil Abdulla; iD, Tarek Rebai; Dhia Hussein Jassim AlDelemi

Authors

Alaa Kamil Abdulla Department of Life Sciences, Faculty of Sciences-Sfax, University of Sfax, Tunisia; 1bDepartment of Medical Biotechnology, Faculty of Biotechnology, University of Al-Qadisiyah, Iraq.
iD, Tarek Rebai PhD: Laboratory Histo-embryology and Cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia
Dhia Hussein Jassim AlDelemi PhD: Department of surgery and obstetrics, Faculty of veterinary medicine, University of Al-Qadisiyah, Iraq.

Keywords:

Platelet-rich plasma, Rabbit, Sperm Parameters, Testis Function

Abstract

This study aims to determine the perfect time for semen collection after intra-testicular platelet-rich plasma to get good and improved semen. Twenty male New Zealand rabbits were used, about 10 to 12 months aged, which randomly divided into two equal groups; the control group and the PRP group which was enriched with 200 μl (4000 × 109 platelets/1 µl) single-dose by intra-testicular injection. The Laboratory analysis of all animals continued at four-time points (4thwk, 6thwk, 8thwk and 10thwk) by measuring the concentration of the Testosterone, Vascular Endothelial Growth Factor (VEGF) and Insulin-like Growth Factor-1 (IGF-1) in the seminal plasma by ELISA technique. Furthermore, the semen analyses which applied by the Computer Assisted Sperm Analysis (CASA) device.